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cell lines hela cells atcc atcc ccl 2tm  (ATCC)


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    ATCC cell lines hela cells atcc atcc ccl 2tm
    Cell Lines Hela Cells Atcc Atcc Ccl 2tm, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+hela+cells+atcc+atcc+ccl+2tm/McCoy/pm38981484-176-209-213
    Average 96 stars, based on 1409 article reviews
    cell lines hela cells atcc atcc ccl 2tm - by Bioz Stars, 2026-10
    96/100 stars

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    Virus:

    Article Title: Combination of compound screening with an animal model identifies pentamidine to prevent Chlamydia trachomatis infection.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ct LPS FITC, clone B410F Invitrogen Cat # MA1-7339; RRID: AB_1016792 Ct LPS, clone 512F Invitrogen Cat # MA5-16287; RRID: AB_2537804 Goat anti-mouse IgG AF680 Invitrogen Cat # A-21058; RRID: AB_2535724 Bacterial and virus strains CTL2P-pGFP:pSW2 Chlamydia Biobank CT401 Ct serovars L2 DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19102 Ct serovars E DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19131 Ct serovars F DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19410 Neisseria gonorrhea ATCC ATCC 49226 Lactobacillus acidophilus ATCC ATCC 4356 Biological samples Healthy adult cervical tissue Medical University of Vienna N/A Chemicals, peptides, and recombinant proteins Azithromycin Synovo N/A C6-NBD Ceramide Focus Biomolecules/Biotrend Cat# 10-5496-1mg Doxycycline Vibravenös Pfizer N/A Dolutegravir MedChemExpress Cat# HY-13238 Cycloheximide Sigma Cat# 01810 Gastrografin Bayer Cat# 80375310 Medroxyprogesterone acetate TCA Cat# M1964 Metergoline Sigma Cat# M3668-500MG Penicillin Sigma Cat# P3032-1MU Pentamidine isethionate Thermo Fisher Scientific Cat# 461860010 Critical commercial assays CellTrace Violet Thermo Fisher Scientific Cat# C34557 7-AAD BD Cat# 559925 LDH-CytoxTM Assay Kit Biolegend Cat# 426401 QIAamp DNA mini kit Qiagen Cat# 51306 Phalloidin AF594 Invitrogen Cat# A12381 Luna Universal Probe qPCR Master Mix New England Biolabs Cat# M3004X Seahorse XF Cell Mito Stress Test Agilent Cat# 103015-100 Experimental models: Cell lines HeLa cells ATCC ATCC CCL-2TM; RRID:CVCL_0030 McCoy cells ATCC ATCC CRL-1696TM; RRID:CVCL_3742 HCT116-SLC19A1-KO-c4 RESOLUTE CE0540-U; RRID:CVCL_D4IH HCT116-Renilla-KO-c1 RESOLUTE CE04CG-T (Continued on next page) e1 Cell Reports Medicine 5, 101643, July 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6JRj Janvier labs/Charles River RRID: IMSR_RJ:C57BL-6JRJ Oligonucleotides Primer Ct 16S DNA IDT F: 50-GGA GGC TGC AGT CGA GAA TCT-30 R: 50-TTA CAA CCC TAG AGC CTT CAT CAC A-30 Probe: 50-[6-FAM]-TCG TCA GAC TTC CGT CCA TTG CGA-[TAMRA]-30 Rodent GAPDH VIC Applied Biosystems Cat# 4308313 Human GAPDH VIC Thermo Fisher Scientific Cat# 4448489 Software and algorithms FlowJo Version 10.8.1 FlowJo https://www.flowjo.com/ Olympus FV31S-SW software Olympus https://www.olympus-lifescience.com/ en/support/downloads/ Prism Version 8 & 9 GraphPad Software https://www.graphpad.com/scientific- software/prism/ BioRender BioRender https://www.biorender.com/

    Article Title: Combination of compound screening with an animal model identifies pentamidine to prevent C hlamydia trachomatis infection
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ct LPS FITC, clone B410F Invitrogen Cat # MA1-7339; RRID: AB_1016792 Ct LPS, clone 512F Invitrogen Cat # MA5-16287; RRID: AB_2537804 Goat anti-mouse IgG AF680 Invitrogen Cat # A-21058; RRID: AB_2535724 Bacterial and virus strains CTL2P-pGFP:pSW2 Chlamydia Biobank CT401 Ct serovars L2 DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19102 Ct serovars E DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19131 Ct serovars F DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19410 Neisseria gonorrhea ATCC ATCC 49226 Lactobacillus acidophilus ATCC ATCC 4356 Biological samples Healthy adult cervical tissue Medical University of Vienna N/A Chemicals, peptides, and recombinant proteins Azithromycin Synovo N/A C6-NBD Ceramide Focus Biomolecules/Biotrend Cat# 10-5496-1mg Doxycycline Vibravenös Pfizer N/A Dolutegravir MedChemExpress Cat# HY-13238 Cycloheximide Sigma Cat# 01810 Gastrografin Bayer Cat# 80375310 Medroxyprogesterone acetate TCA Cat# M1964 Metergoline Sigma Cat# M3668-500MG Penicillin Sigma Cat# P3032-1MU Pentamidine isethionate Thermo Fisher Scientific Cat# 461860010 Critical commercial assays CellTrace Violet Thermo Fisher Scientific Cat# C34557 7-AAD BD Cat# 559925 LDH-CytoxTM Assay Kit Biolegend Cat# 426401 QIAamp DNA mini kit Qiagen Cat# 51306 Phalloidin AF594 Invitrogen Cat# A12381 Luna Universal Probe qPCR Master Mix New England Biolabs Cat# M3004X Seahorse XF Cell Mito Stress Test Agilent Cat# 103015-100 Experimental models: Cell lines HeLa cells ATCC ATCC CCL-2TM; RRID:CVCL_0030 McCoy cells ATCC ATCC CRL-1696TM; RRID:CVCL_3742 HCT116-SLC19A1-KO-c4 RESOLUTE CE0540-U; RRID:CVCL_D4IH HCT116-Renilla-KO-c1 RESOLUTE CE04CG-T (Continued on next page) e1 Cell Reports Medicine 5, 101643, July 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6JRj Janvier labs/Charles River RRID: IMSR_RJ:C57BL-6JRJ Oligonucleotides Primer Ct 16S DNA IDT F: 50-GGA GGC TGC AGT CGA GAA TCT-30 R: 50-TTA CAA CCC TAG AGC CTT CAT CAC A-30 Probe: 50-[6-FAM]-TCG TCA GAC TTC CGT CCA TTG CGA-[TAMRA]-30 Rodent GAPDH VIC Applied Biosystems Cat# 4308313 Human GAPDH VIC Thermo Fisher Scientific Cat# 4448489 Software and algorithms FlowJo Version 10.8.1 FlowJo https://www.flowjo.com/ Olympus FV31S-SW software Olympus https://www.olympus-lifescience.com/ en/support/downloads/ Prism Version 8 & 9 GraphPad Software https://www.graphpad.com/scientific- software/prism/ BioRender BioRender https://www.biorender.com/ Article ll OPEN ACCESS

    Cell Culture:

    Article Title: Combination of compound screening with an animal model identifies pentamidine to prevent Chlamydia trachomatis infection.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ct LPS FITC, clone B410F Invitrogen Cat # MA1-7339; RRID: AB_1016792 Ct LPS, clone 512F Invitrogen Cat # MA5-16287; RRID: AB_2537804 Goat anti-mouse IgG AF680 Invitrogen Cat # A-21058; RRID: AB_2535724 Bacterial and virus strains CTL2P-pGFP:pSW2 Chlamydia Biobank CT401 Ct serovars L2 DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19102 Ct serovars E DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19131 Ct serovars F DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19410 Neisseria gonorrhea ATCC ATCC 49226 Lactobacillus acidophilus ATCC ATCC 4356 Biological samples Healthy adult cervical tissue Medical University of Vienna N/A Chemicals, peptides, and recombinant proteins Azithromycin Synovo N/A C6-NBD Ceramide Focus Biomolecules/Biotrend Cat# 10-5496-1mg Doxycycline Vibravenös Pfizer N/A Dolutegravir MedChemExpress Cat# HY-13238 Cycloheximide Sigma Cat# 01810 Gastrografin Bayer Cat# 80375310 Medroxyprogesterone acetate TCA Cat# M1964 Metergoline Sigma Cat# M3668-500MG Penicillin Sigma Cat# P3032-1MU Pentamidine isethionate Thermo Fisher Scientific Cat# 461860010 Critical commercial assays CellTrace Violet Thermo Fisher Scientific Cat# C34557 7-AAD BD Cat# 559925 LDH-CytoxTM Assay Kit Biolegend Cat# 426401 QIAamp DNA mini kit Qiagen Cat# 51306 Phalloidin AF594 Invitrogen Cat# A12381 Luna Universal Probe qPCR Master Mix New England Biolabs Cat# M3004X Seahorse XF Cell Mito Stress Test Agilent Cat# 103015-100 Experimental models: Cell lines HeLa cells ATCC ATCC CCL-2TM; RRID:CVCL_0030 McCoy cells ATCC ATCC CRL-1696TM; RRID:CVCL_3742 HCT116-SLC19A1-KO-c4 RESOLUTE CE0540-U; RRID:CVCL_D4IH HCT116-Renilla-KO-c1 RESOLUTE CE04CG-T (Continued on next page) e1 Cell Reports Medicine 5, 101643, July 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6JRj Janvier labs/Charles River RRID: IMSR_RJ:C57BL-6JRJ Oligonucleotides Primer Ct 16S DNA IDT F: 50-GGA GGC TGC AGT CGA GAA TCT-30 R: 50-TTA CAA CCC TAG AGC CTT CAT CAC A-30 Probe: 50-[6-FAM]-TCG TCA GAC TTC CGT CCA TTG CGA-[TAMRA]-30 Rodent GAPDH VIC Applied Biosystems Cat# 4308313 Human GAPDH VIC Thermo Fisher Scientific Cat# 4448489 Software and algorithms FlowJo Version 10.8.1 FlowJo https://www.flowjo.com/ Olympus FV31S-SW software Olympus https://www.olympus-lifescience.com/ en/support/downloads/ Prism Version 8 & 9 GraphPad Software https://www.graphpad.com/scientific- software/prism/ BioRender BioRender https://www.biorender.com/

    Article Title: Combination of compound screening with an animal model identifies pentamidine to prevent C hlamydia trachomatis infection
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ct LPS FITC, clone B410F Invitrogen Cat # MA1-7339; RRID: AB_1016792 Ct LPS, clone 512F Invitrogen Cat # MA5-16287; RRID: AB_2537804 Goat anti-mouse IgG AF680 Invitrogen Cat # A-21058; RRID: AB_2535724 Bacterial and virus strains CTL2P-pGFP:pSW2 Chlamydia Biobank CT401 Ct serovars L2 DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19102 Ct serovars E DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19131 Ct serovars F DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19410 Neisseria gonorrhea ATCC ATCC 49226 Lactobacillus acidophilus ATCC ATCC 4356 Biological samples Healthy adult cervical tissue Medical University of Vienna N/A Chemicals, peptides, and recombinant proteins Azithromycin Synovo N/A C6-NBD Ceramide Focus Biomolecules/Biotrend Cat# 10-5496-1mg Doxycycline Vibravenös Pfizer N/A Dolutegravir MedChemExpress Cat# HY-13238 Cycloheximide Sigma Cat# 01810 Gastrografin Bayer Cat# 80375310 Medroxyprogesterone acetate TCA Cat# M1964 Metergoline Sigma Cat# M3668-500MG Penicillin Sigma Cat# P3032-1MU Pentamidine isethionate Thermo Fisher Scientific Cat# 461860010 Critical commercial assays CellTrace Violet Thermo Fisher Scientific Cat# C34557 7-AAD BD Cat# 559925 LDH-CytoxTM Assay Kit Biolegend Cat# 426401 QIAamp DNA mini kit Qiagen Cat# 51306 Phalloidin AF594 Invitrogen Cat# A12381 Luna Universal Probe qPCR Master Mix New England Biolabs Cat# M3004X Seahorse XF Cell Mito Stress Test Agilent Cat# 103015-100 Experimental models: Cell lines HeLa cells ATCC ATCC CCL-2TM; RRID:CVCL_0030 McCoy cells ATCC ATCC CRL-1696TM; RRID:CVCL_3742 HCT116-SLC19A1-KO-c4 RESOLUTE CE0540-U; RRID:CVCL_D4IH HCT116-Renilla-KO-c1 RESOLUTE CE04CG-T (Continued on next page) e1 Cell Reports Medicine 5, 101643, July 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6JRj Janvier labs/Charles River RRID: IMSR_RJ:C57BL-6JRJ Oligonucleotides Primer Ct 16S DNA IDT F: 50-GGA GGC TGC AGT CGA GAA TCT-30 R: 50-TTA CAA CCC TAG AGC CTT CAT CAC A-30 Probe: 50-[6-FAM]-TCG TCA GAC TTC CGT CCA TTG CGA-[TAMRA]-30 Rodent GAPDH VIC Applied Biosystems Cat# 4308313 Human GAPDH VIC Thermo Fisher Scientific Cat# 4448489 Software and algorithms FlowJo Version 10.8.1 FlowJo https://www.flowjo.com/ Olympus FV31S-SW software Olympus https://www.olympus-lifescience.com/ en/support/downloads/ Prism Version 8 & 9 GraphPad Software https://www.graphpad.com/scientific- software/prism/ BioRender BioRender https://www.biorender.com/ Article ll OPEN ACCESS

    Recombinant:

    Article Title: Combination of compound screening with an animal model identifies pentamidine to prevent Chlamydia trachomatis infection.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ct LPS FITC, clone B410F Invitrogen Cat # MA1-7339; RRID: AB_1016792 Ct LPS, clone 512F Invitrogen Cat # MA5-16287; RRID: AB_2537804 Goat anti-mouse IgG AF680 Invitrogen Cat # A-21058; RRID: AB_2535724 Bacterial and virus strains CTL2P-pGFP:pSW2 Chlamydia Biobank CT401 Ct serovars L2 DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19102 Ct serovars E DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19131 Ct serovars F DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19410 Neisseria gonorrhea ATCC ATCC 49226 Lactobacillus acidophilus ATCC ATCC 4356 Biological samples Healthy adult cervical tissue Medical University of Vienna N/A Chemicals, peptides, and recombinant proteins Azithromycin Synovo N/A C6-NBD Ceramide Focus Biomolecules/Biotrend Cat# 10-5496-1mg Doxycycline Vibravenös Pfizer N/A Dolutegravir MedChemExpress Cat# HY-13238 Cycloheximide Sigma Cat# 01810 Gastrografin Bayer Cat# 80375310 Medroxyprogesterone acetate TCA Cat# M1964 Metergoline Sigma Cat# M3668-500MG Penicillin Sigma Cat# P3032-1MU Pentamidine isethionate Thermo Fisher Scientific Cat# 461860010 Critical commercial assays CellTrace Violet Thermo Fisher Scientific Cat# C34557 7-AAD BD Cat# 559925 LDH-CytoxTM Assay Kit Biolegend Cat# 426401 QIAamp DNA mini kit Qiagen Cat# 51306 Phalloidin AF594 Invitrogen Cat# A12381 Luna Universal Probe qPCR Master Mix New England Biolabs Cat# M3004X Seahorse XF Cell Mito Stress Test Agilent Cat# 103015-100 Experimental models: Cell lines HeLa cells ATCC ATCC CCL-2TM; RRID:CVCL_0030 McCoy cells ATCC ATCC CRL-1696TM; RRID:CVCL_3742 HCT116-SLC19A1-KO-c4 RESOLUTE CE0540-U; RRID:CVCL_D4IH HCT116-Renilla-KO-c1 RESOLUTE CE04CG-T (Continued on next page) e1 Cell Reports Medicine 5, 101643, July 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6JRj Janvier labs/Charles River RRID: IMSR_RJ:C57BL-6JRJ Oligonucleotides Primer Ct 16S DNA IDT F: 50-GGA GGC TGC AGT CGA GAA TCT-30 R: 50-TTA CAA CCC TAG AGC CTT CAT CAC A-30 Probe: 50-[6-FAM]-TCG TCA GAC TTC CGT CCA TTG CGA-[TAMRA]-30 Rodent GAPDH VIC Applied Biosystems Cat# 4308313 Human GAPDH VIC Thermo Fisher Scientific Cat# 4448489 Software and algorithms FlowJo Version 10.8.1 FlowJo https://www.flowjo.com/ Olympus FV31S-SW software Olympus https://www.olympus-lifescience.com/ en/support/downloads/ Prism Version 8 & 9 GraphPad Software https://www.graphpad.com/scientific- software/prism/ BioRender BioRender https://www.biorender.com/

    Article Title: Combination of compound screening with an animal model identifies pentamidine to prevent C hlamydia trachomatis infection
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ct LPS FITC, clone B410F Invitrogen Cat # MA1-7339; RRID: AB_1016792 Ct LPS, clone 512F Invitrogen Cat # MA5-16287; RRID: AB_2537804 Goat anti-mouse IgG AF680 Invitrogen Cat # A-21058; RRID: AB_2535724 Bacterial and virus strains CTL2P-pGFP:pSW2 Chlamydia Biobank CT401 Ct serovars L2 DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19102 Ct serovars E DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19131 Ct serovars F DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19410 Neisseria gonorrhea ATCC ATCC 49226 Lactobacillus acidophilus ATCC ATCC 4356 Biological samples Healthy adult cervical tissue Medical University of Vienna N/A Chemicals, peptides, and recombinant proteins Azithromycin Synovo N/A C6-NBD Ceramide Focus Biomolecules/Biotrend Cat# 10-5496-1mg Doxycycline Vibravenös Pfizer N/A Dolutegravir MedChemExpress Cat# HY-13238 Cycloheximide Sigma Cat# 01810 Gastrografin Bayer Cat# 80375310 Medroxyprogesterone acetate TCA Cat# M1964 Metergoline Sigma Cat# M3668-500MG Penicillin Sigma Cat# P3032-1MU Pentamidine isethionate Thermo Fisher Scientific Cat# 461860010 Critical commercial assays CellTrace Violet Thermo Fisher Scientific Cat# C34557 7-AAD BD Cat# 559925 LDH-CytoxTM Assay Kit Biolegend Cat# 426401 QIAamp DNA mini kit Qiagen Cat# 51306 Phalloidin AF594 Invitrogen Cat# A12381 Luna Universal Probe qPCR Master Mix New England Biolabs Cat# M3004X Seahorse XF Cell Mito Stress Test Agilent Cat# 103015-100 Experimental models: Cell lines HeLa cells ATCC ATCC CCL-2TM; RRID:CVCL_0030 McCoy cells ATCC ATCC CRL-1696TM; RRID:CVCL_3742 HCT116-SLC19A1-KO-c4 RESOLUTE CE0540-U; RRID:CVCL_D4IH HCT116-Renilla-KO-c1 RESOLUTE CE04CG-T (Continued on next page) e1 Cell Reports Medicine 5, 101643, July 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6JRj Janvier labs/Charles River RRID: IMSR_RJ:C57BL-6JRJ Oligonucleotides Primer Ct 16S DNA IDT F: 50-GGA GGC TGC AGT CGA GAA TCT-30 R: 50-TTA CAA CCC TAG AGC CTT CAT CAC A-30 Probe: 50-[6-FAM]-TCG TCA GAC TTC CGT CCA TTG CGA-[TAMRA]-30 Rodent GAPDH VIC Applied Biosystems Cat# 4308313 Human GAPDH VIC Thermo Fisher Scientific Cat# 4448489 Software and algorithms FlowJo Version 10.8.1 FlowJo https://www.flowjo.com/ Olympus FV31S-SW software Olympus https://www.olympus-lifescience.com/ en/support/downloads/ Prism Version 8 & 9 GraphPad Software https://www.graphpad.com/scientific- software/prism/ BioRender BioRender https://www.biorender.com/ Article ll OPEN ACCESS

    Real-time Polymerase Chain Reaction:

    Article Title: Combination of compound screening with an animal model identifies pentamidine to prevent Chlamydia trachomatis infection.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ct LPS FITC, clone B410F Invitrogen Cat # MA1-7339; RRID: AB_1016792 Ct LPS, clone 512F Invitrogen Cat # MA5-16287; RRID: AB_2537804 Goat anti-mouse IgG AF680 Invitrogen Cat # A-21058; RRID: AB_2535724 Bacterial and virus strains CTL2P-pGFP:pSW2 Chlamydia Biobank CT401 Ct serovars L2 DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19102 Ct serovars E DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19131 Ct serovars F DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19410 Neisseria gonorrhea ATCC ATCC 49226 Lactobacillus acidophilus ATCC ATCC 4356 Biological samples Healthy adult cervical tissue Medical University of Vienna N/A Chemicals, peptides, and recombinant proteins Azithromycin Synovo N/A C6-NBD Ceramide Focus Biomolecules/Biotrend Cat# 10-5496-1mg Doxycycline Vibravenös Pfizer N/A Dolutegravir MedChemExpress Cat# HY-13238 Cycloheximide Sigma Cat# 01810 Gastrografin Bayer Cat# 80375310 Medroxyprogesterone acetate TCA Cat# M1964 Metergoline Sigma Cat# M3668-500MG Penicillin Sigma Cat# P3032-1MU Pentamidine isethionate Thermo Fisher Scientific Cat# 461860010 Critical commercial assays CellTrace Violet Thermo Fisher Scientific Cat# C34557 7-AAD BD Cat# 559925 LDH-CytoxTM Assay Kit Biolegend Cat# 426401 QIAamp DNA mini kit Qiagen Cat# 51306 Phalloidin AF594 Invitrogen Cat# A12381 Luna Universal Probe qPCR Master Mix New England Biolabs Cat# M3004X Seahorse XF Cell Mito Stress Test Agilent Cat# 103015-100 Experimental models: Cell lines HeLa cells ATCC ATCC CCL-2TM; RRID:CVCL_0030 McCoy cells ATCC ATCC CRL-1696TM; RRID:CVCL_3742 HCT116-SLC19A1-KO-c4 RESOLUTE CE0540-U; RRID:CVCL_D4IH HCT116-Renilla-KO-c1 RESOLUTE CE04CG-T (Continued on next page) e1 Cell Reports Medicine 5, 101643, July 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6JRj Janvier labs/Charles River RRID: IMSR_RJ:C57BL-6JRJ Oligonucleotides Primer Ct 16S DNA IDT F: 50-GGA GGC TGC AGT CGA GAA TCT-30 R: 50-TTA CAA CCC TAG AGC CTT CAT CAC A-30 Probe: 50-[6-FAM]-TCG TCA GAC TTC CGT CCA TTG CGA-[TAMRA]-30 Rodent GAPDH VIC Applied Biosystems Cat# 4308313 Human GAPDH VIC Thermo Fisher Scientific Cat# 4448489 Software and algorithms FlowJo Version 10.8.1 FlowJo https://www.flowjo.com/ Olympus FV31S-SW software Olympus https://www.olympus-lifescience.com/ en/support/downloads/ Prism Version 8 & 9 GraphPad Software https://www.graphpad.com/scientific- software/prism/ BioRender BioRender https://www.biorender.com/

    Article Title: Combination of compound screening with an animal model identifies pentamidine to prevent C hlamydia trachomatis infection
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ct LPS FITC, clone B410F Invitrogen Cat # MA1-7339; RRID: AB_1016792 Ct LPS, clone 512F Invitrogen Cat # MA5-16287; RRID: AB_2537804 Goat anti-mouse IgG AF680 Invitrogen Cat # A-21058; RRID: AB_2535724 Bacterial and virus strains CTL2P-pGFP:pSW2 Chlamydia Biobank CT401 Ct serovars L2 DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19102 Ct serovars E DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19131 Ct serovars F DSMZ-German Collection of Microorganisms and Cell Culture GmBH DSM 19410 Neisseria gonorrhea ATCC ATCC 49226 Lactobacillus acidophilus ATCC ATCC 4356 Biological samples Healthy adult cervical tissue Medical University of Vienna N/A Chemicals, peptides, and recombinant proteins Azithromycin Synovo N/A C6-NBD Ceramide Focus Biomolecules/Biotrend Cat# 10-5496-1mg Doxycycline Vibravenös Pfizer N/A Dolutegravir MedChemExpress Cat# HY-13238 Cycloheximide Sigma Cat# 01810 Gastrografin Bayer Cat# 80375310 Medroxyprogesterone acetate TCA Cat# M1964 Metergoline Sigma Cat# M3668-500MG Penicillin Sigma Cat# P3032-1MU Pentamidine isethionate Thermo Fisher Scientific Cat# 461860010 Critical commercial assays CellTrace Violet Thermo Fisher Scientific Cat# C34557 7-AAD BD Cat# 559925 LDH-CytoxTM Assay Kit Biolegend Cat# 426401 QIAamp DNA mini kit Qiagen Cat# 51306 Phalloidin AF594 Invitrogen Cat# A12381 Luna Universal Probe qPCR Master Mix New England Biolabs Cat# M3004X Seahorse XF Cell Mito Stress Test Agilent Cat# 103015-100 Experimental models: Cell lines HeLa cells ATCC ATCC CCL-2TM; RRID:CVCL_0030 McCoy cells ATCC ATCC CRL-1696TM; RRID:CVCL_3742 HCT116-SLC19A1-KO-c4 RESOLUTE CE0540-U; RRID:CVCL_D4IH HCT116-Renilla-KO-c1 RESOLUTE CE04CG-T (Continued on next page) e1 Cell Reports Medicine 5, 101643, July 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6JRj Janvier labs/Charles River RRID: IMSR_RJ:C57BL-6JRJ Oligonucleotides Primer Ct 16S DNA IDT F: 50-GGA GGC TGC AGT CGA GAA TCT-30 R: 50-TTA CAA CCC TAG AGC CTT CAT CAC A-30 Probe: 50-[6-FAM]-TCG TCA GAC TTC CGT CCA TTG CGA-[TAMRA]-30 Rodent GAPDH VIC Applied Biosystems Cat# 4308313 Human GAPDH VIC Thermo Fisher Scientific Cat# 4448489 Software and algorithms FlowJo Version 10.8.1 FlowJo https://www.flowjo.com/ Olympus FV31S-SW software Olympus https://www.olympus-lifescience.com/ en/support/downloads/ Prism Version 8 & 9 GraphPad Software https://www.graphpad.com/scientific- software/prism/ BioRender BioRender https://www.biorender.com/ Article ll OPEN ACCESS



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